Converging on Cancer Seminar Series – Engineering and the Physical Sciences to Advance Cancer Research (Physics and Cancer) | Events


As part of its mission to facilitate collaboration between traditionally separate and distinct disciplines to innovate new ways to address the challenges of cancer – the Cancer Research UK Convergence Science Center will be bringing you a series of webinars over the coming months featuring the expertise and technology of Imperial chemists, bio-engineers, physicists and mathematicians. Please join us in inspiring thinking about how these new technologies could be used to shed light on unresolved issues in cancer biology and bring innovative treatments to cancer patients more quickly.

Professor Paul French – Department of Physics, Imperial College London

Multidimensional fluorescence imaging of cancer


Dr Chris Dunsby – Department of Physics, Imperial College London

High Speed ​​3D Fluorescence Microscopy High Light Sheet Content


To receive information on how to access this event, please email [email protected]

Note: This webinar is exclusively for colleagues at the Institute of Cancer Research, Imperial College London, Royal Marsden Hospital and Imperial College Healthcare.

About the speakers and presentations:

Professor Paul French

In this talk, Professor French will discuss the development of instrumentation including microscopy, high content analysis, endoscopy and tomography applied to unmarked imaging of cancer and the exploration of molecular processes under -jacent. This will include super-resolved microscopy, lifetime fluorescence imaging, and FRET.

Paul French is Professor in the Physics Department at Imperial Oil and is currently Associate Dean (Research) in the Faculty of Natural Sciences. His research group is based in the Department of Physics at Imperial Oil and also has a satellite laboratory at the Francis Crick Institute. Research has evolved from the physics of ultrafast dyes and solid state lasers to biomedical optics for applications in molecular cell biology, drug discovery and clinical diagnostics. Its current portfolio includes the development and application of multidimensional fluorescence imaging technology for super-resolved microscopy, automated high-content analysis, endoscopy and tomography, with open source approaches to instrumentation. , including hardware, data acquisition and analysis.

Dr Chris Dunsby

Light sheet fluorescence microscopy (LSFM) offers low photobleaching and out-of-plane phototoxicity, but typically requires two microscope objective lenses oriented 90 ° to each other – one for excitation for fluorescence and one for fluorescence detection – making it more difficult to image samples prepared using conventional mounting methods. In this talk, Dr Dunsby will talk about Plane Oblique Microscopy (OPM), which is a type of LSFM that was developed in his lab that uses a single large numerical aperture microscope objective to provide both excitation and fluorescence detection while retaining the advantages of LSFM. – enabling it to provide high speed 3D imaging for a range of applications on a conventional fluorescence microscope frame. The speed of OPM imaging can be applied to image a single sample at video volumetric imaging rates. It can also be used to enable accelerated 3D imaging of sample arrays arranged in multiwell plates. This presentation will present application examples of OPM for high speed 3D imaging of isolated cardiomyocytes, as well as examples where the system is applied to study 3D multicellular spheroid and organoid arrays under several conditions and over time.

Chris Dunsby is working on the development of quantitative fluorescence imaging techniques for applications in biomedicine, including Förster’s resonant energy transfer microscopy, automated multiwell plate imaging, and lifetime fluorescence imaging (FLIM) . He has also worked on super-resolution fluorescence imaging techniques and collaborates with colleagues in bioengineering to translate optical methods to obtain super-resolved ultrasound imaging. He invented and patented oblique plane microscopy, which is a form of light sheet fluorescence microscopy that can be performed on a conventional microscope frame to provide high speed 2D and 3D imaging. He has also led projects for the development and application of multiphoton imaging and FLIM for clinical applications and has developed new multiphoton endoscopes.

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